Saurabh bhatia, kiran sharma, in modern applications of plant biotechnology in pharmaceutical sciences, 2015. Download somaclonal variation in crop improvement i. A case study with olive 125 whereas in vitro the loss of cellular control, which gives rise to disorganised growth, is a characteristic of somaclonal variation 6,19. The hawaian sugar planters association experimental station recorded genetic variation among sugarcane plants regenerated from tissue culture. Find powerpoint presentations and slides using the power of, find free presentations research about somaclonal variation ppt. Read this article to learn about the basis, isolation and limitations of somaclonal variations and also factors affecting the production of somaclonal variants. In vitro studies on somaclonal variation and induced. Cluster analysis, principal component analysis, somaclonal variations, sugarcane, tissue culture.
Somatic embryogenesis somatic hybrid somaclonal variation crop improvement. The variation in invitro culture is called as a invitro variation b mutation c somaclonal variation d all of these 14. Pdf in vitro propagation of sugarcane for certified seed. The occurrence of subtle somaclonal variation is a drawback for both in. Somaclonal variation and in vitro mutation induction. Sugarcane variety co 94012 is a new variety released in india through the use of somaclonal variation. Detection of somaclonal variation in micropropagated plants of sugarcane and scmv screening through elisa. Transformation of foreign gene in sugarcane variety isd 28 using agrobacterummediated method. Somaclonal variation is the introduction of genetic variation in in vitro cultured cells and. The genetic variations found in the in vitro cultured cells are collectively referred to as somaclonal variations. Somaclonal variation in crop improvement springerlink. These clones have different discard percentages and were categorized according to the in vitro visual frequency of somaclonal variation symptoms abnormal in vitro plantlets in two groups. Variations in number and structure of chromosomes are commonly observed. There are exmaples in wheat, maize, celery, tomato, banana, oil palm, sugarcane, potato, oats, rice, lettuce, tobacco, carrot, barley and alfalfa.
The usefulness of variation was first demonstrated through the recovery of disease resistant plants in potato resistance against late blight and early blight and sugarcane resistance against eyespot disease, fiji disease and downy. Genetic variation found to occur between somaclones in plant tissue cultures was called somaclonal variation. Development of somaclones of sugarcane with increasing sugar contents reported by 5,6. Determining the genetic stability of micropropagated. Variations were recorded based on cytogenetic, morphological and enzyme profiles. If youre looking for a free download links of somaclonal variation in crop improvement i.
Pdf in vitro regeneration, detection of somaclonal. Yld of sugarcane is caused by sugarcane yellow leaf virus. View and download powerpoint presentations on somaclonal variation ppt. Although the direct formation of plant structures from meristem cultures, without any. Thousands of people are now gout free simply from eating foods found in their local supermarket now im one of them. Somaclonal variation is a tool that can be used by plant breeders. Pdf somaclonal variation as a tool for crop improvement.
This technique is also used to make disease free plants in sugarcane by somaclonal variation. An assessment of somaclonal variation in micropropagated plants of. Somaclonal variation can pose a serious problem in any micropropagation program, where it is highly desirable to produce truetotype plant material. The plants derived from such cells are referred to somaclones. Due to limited genetic system andor narrow genetic base, somaclonal variation is more rewarding in sugarcane. On the other hand, somaclonal variation has provided a new and alternative tool to the breeders for obtaining genetic variability relatively rapidly and without sophisticated technology in. Assessment of somaclonal variation during sugarcane. An engineering view to micropropagation and generation of true to type and pathogenfree plants.
Transfers were made at 30day intervals for 1, 2 or 3 subcultures. Somaclonal variation can be manifested as either somatically or meiotically stable events. Somaclonal variants resistant to sugarcane mosaic virus scmv were obtained from susceptible sugarcane cv pr62258 through somatic embryogenesis by increasing the number of subcultures of the embryogenic callus tissue in ms medium with 3 mgl 2,4dichlorophenoxyacetic acid. In the present investigation, optimal media compositions for enhancing the callus induction and subsequent plant regeneration were worked out in sugarcane saccharum spp. A study was conducted from 2006 to 2008 to assess the variability arising from callus regeneration and its vegetative transmission in a. Multiple choice questions on plant biotechnology mcq. Pdf assessment of genetic variability in somaclonal. Dna analysis to detect genetic change in sugarcane during tissue culture. Tissue culture is an efficient method of clonal propagation. Pdf eight genotypes of saccharum officinarum were crossed with saccharum spontaneum and 14 genotypes of s.
Because there is a chance of result of unwanted offtypes that reduces the commercial value. Somaclonal variations may reduce two years the time required for the release of new variety compared to mutation breeding. Micropropagation of sugarcane is important to obtain pathogen free plants, genetically homogeneous and invigorate. In vitro generation of somaclonal variant plants of sugarcane saccharum spp. The field grown somaclones were characterized for yield and quality. The occurrence of somaclonal variation is both a boon and bane. Somaclonal variation is an important aspect of tissue culture technology and widely recommended for crop improvement. Seed multiplication of newly released varieties of sugarcane is one of the major. These somaclonal variations are mainly caused by newly generated mutations arising from tissue culture process sato et al. Haploid plants are produced in large numbers by a anther culture b ovary culture c both a and b d embryo culture 15. Somaclonal variation in sugarcane various factors responsible for somaclonal variation which include karyotype changes, cryptic changes associated with chromosome rearrangement,transposable elements, somatic gene rearrangements,gene amplification and depletion, somatic crossing over and sisterchromatoid exchanges. In vitro generation of somaclonal variant plants of.
Somaclonal variation includes aneuploids, sterile plants and morphological variants, sometimes involving traits of economic importance in case of crop plants. Somaclonal variation and its application in ornamentals plants. Similarly, genetically transformed cells are carefully screened to avoid unwanted and unintended somaclonal variations so that the commercially released clones have only beneficial effects. In addition, the aim of this chapter is to report, from the laboratory to the. Pdf somaclonal variation in sugarcane through tissue. Somaclonal variation in sugarcane through tissue culture and evaluation for quantitative and quality traits.
Somaclonal variation refers to the genetic and epigenetic changes in plants regenerated from plant tissue culture. Regeneration and evaluation of somaclones of sugarcane variety. Somaclonal variation was first reported in sugarcane plant derived from cell culture in 1969 by the researchers at the hawaiian sugar planters association experiment station15,16,17. Somaclonal variation, therefore, can arise from somatic mutations already present in the donor plant karp 1994. Eight genotypes of saccharum officinarum were crossed with saccharum spontaneum and 14 genotypes of s. Sources of variations detected in plant tissue culture. Net somaclonal variation for sugarcane improvementdownload somaclonal variation for sugarcane improvement ebook pdf. Plant tissue culture or micropropagation technique is the rapid method to multiply newly released cultivar in limited time. Application of somaclonal variation in crop improvement programme was established in sugarcane. Somaclonal variation in sugarcane through tissue culture and evaluation for quantitative and quality traits article pdf available in euphytica 1681. Somaclonal variations and their applications in horticultural crops.
Micropropagation for largescale sugarcane production using a temporary immersion system tis is described. In vitro induction and characterization of somaclonal. Somaclonal variation and in vitro mutation induction have been used in sugarcane, banana and potato, with the objective of improving specific characters, mainly the resistance to diseases and other defects that limit the use of some important commercial varieties. Somaclonal variation is the variation seen in plants that have been produced by plant tissue culture. During plant propagation process under laboratory conditions, the genomic shock is induced which can result in the activation of various transposons, retrotransposons, chromosomal changes, methylation and demethylation of dna leading. Performance of sugarcane somaclones under field condition. Sugarcane improvement through biotechnology authorstream. Somaclonal variations have largely contributed towards the development of disease resistance in many crops e. Dna methylation in sugarcane somaclonal variants assessed. Selected crops somaclonal variants, with increased disease resistance developed, without in vitro selection and within vitro selection are respectively given in tables 46. Chromosomal rearrangements are an important source of this variation.
Somaclonal variation for sugarcane improvement springerlink. Somaclonal variation free download as powerpoint presentation. Proc int soc sugar cane t echnol 157 kumar p agarwal a t iwari ak et al 2012 possibilities of development of red rot. Genetic variations in plants that have been produced by plant tissue culture and can be detected as genetic or phenotypic traits. Applications of somaclonal variations biology discussion. Nevertheless, progress in the adoption of such technology in the generation of. These 39 hybrids were raised in the field and used as donor clones for in vitro culture studies. Avoiding the somaclonal variation in the production of transgenic sugarcane plants. Detection of somaclonal variation in micropropagated. In vitro regeneration, detection of somaclonal variation and screening for mosaic virus in sugarcane saccharum spp. Somaclonal variation and crop improvement larkin and scowcroft 1981 proposed the term somaclone to describe the plants originating from any type of tissue culture.
Identification of somaclonal variants of sugarcane. Pdf somaclonal variation in sugarcane through tissue culture and. It can be concluded from the study that somaclonal variations can create highly diverse populations of sugarcane for evaluation in cane breeding programs. Genetic variability is an important parameter for plant breeders in any con. Assessment of somaclonal variation in sugarcane semantic scholar. In this study, using intersimple sequence repeat issr molecular markers, the somaclonal variation during micropropagation of sugarcane using temporary immersion bioreactors tibs was evaluated. Type and concentration of applied plant growth regulators pgr optimal concentration and precise ratios of auxins and cytokinins is essential for efficient micropropagation skoog and miller 1957. Crop improvement by conventional method in vegetatively propagated crops like sugarcane is very difficult due to its narrow. Detection of somaclonal variation in micropropagated plants of.
The term somaclonal variation is a phenomenon of broad taxonomic occurrence, reported for species of different ploidy levels, and for outcrossing and inbreeding, vegetatively and seed propagated, and cultivated and non. Thus, the purpose of this study was to investigate the existence of somaclonal variation in sugarcane varieties from. The micropropagation procedure is divided into stages for the sake of better understanding. Advantages somaclonal variations are stable and occur at high frequencies. The plant regeneration from callus are capable of producing somaclonal variants for different traits like high. Somaclonal variation has been observed in more than 30 species, including most important crops. Somaclonal variation and induced mutations in crop improvement. Cybrids are a nuclear hybrids b hybrid plants derived from cross pollination c cytoplasmic hybrids. Assessment of genetic variability in somaclonal population of sugarcane article pdf available in pakistan journal of botany 466. Somaclonal variation caused by the process of tissue culture is also called tissue culture induced variation to more speci. No meds, doctors or procedures three years of pain gone in weeks. Somaclonal variation and its application in ornamentals.